Minichromosome maintenance

MCM2/3/5 family

Structure of MCM from archaeal M. Thermoautotrophicum.[1]
Identifiers
Symbol MCM
Pfam PF00493
Pfam clan CL0023
InterPro IPR001208
SMART SM00350
PROSITE PDOC00662
Potential role of Cdc6 at the initiation of DNA replication.[2]

Minichromosome maintenance protein complex (MCM) is a eukaryotic DNA helicase complex required for the process of DNA replication, specifically the formation and elongation of the replication fork. It is a hexamer of six related polypeptides (MCM2 through MCM7) that form a ring structure.[3][4][5] MCM is a component of the pre-replication complex that forms at the eukaryotic origin of replication.

Control of MCM activity in the cell is partly achieved by its sumoylation. During cell cycle levels of chromatin-bound MCM sumoylation oscillate, loss of MCM sumoylation occurs immediately before DNA replication[6] Increased expression level of MCM proteins is observed in cells of different cancer types. MCMs could be useful markers of cancer cell proliferation.[7][8]

See also

References

  1. Fletcher RJ, Bishop BE, Leon RP, Sclafani RA, Ogata CM, Chen XS (March 2003). "The structure and function of MCM from archaeal M. Thermoautotrophicum". Nat. Struct. Biol. 10 (3): 160–7. doi:10.1038/nsb893. PMID 12548282.
  2. Borlado LR, Méndez J (February 2008). "CDC6: from DNA replication to cell cycle checkpoints and oncogenesis". Carcinogenesis. 29 (2): 237–43. doi:10.1093/carcin/bgm268. PMID 18048387.
  3. 1 2 Cortez D, Glick G, Elledge SJ (July 2004). "Minichromosome maintenance proteins are direct targets of the ATM and ATR checkpoint kinases". Proc. Natl. Acad. Sci. U.S.A. 101 (27): 10078–83. doi:10.1073/pnas.0403410101. PMC 454167Freely accessible. PMID 15210935.
  4. Carpentieri F, De Felice M, De Falco M, Rossi M, Pisani FM (April 2002). "Physical and functional interaction between the mini-chromosome maintenance-like DNA helicase and the single-stranded DNA binding protein from the crenarchaeon Sulfolobus solfataricus". J. Biol. Chem. 277 (14): 12118–27. doi:10.1074/jbc.M200091200. PMID 11821426.
  5. Brewster, Aaron; Chen, Xiaojiang (June 2010). "Insights into MCM functional mechanism: lessons learned from the archaeal MCM complex". Crit Rev Biochem Mol Biol. 45 (3): 243–256. doi:10.3109/10409238.2010.484836. PMC 2953368Freely accessible. PMID 20441442.
  6. Baumann, Kim (2016). "DNA replication: SUMO wrestling to get the timing right". Nature Reviews Molecular Cell Biology. 17 (3): 134–135. doi:10.1038/nrm.2016.15. ISSN 1471-0072.
  7. Valverde, Ludmila de F.; de Freitas, Raíza D.; Pereira, Thiago de A.; de Resende, Marina F.; Agra, Ivan M. G.; Dos Santos, Jean N.; Dos Reis, Mitermayer G.; Sales, Caroline B. S.; Gurgel Rocha, Clarissa A. (2016-06-02). "MCM3: A Novel Proliferation Marker in Oral Squamous Cell Carcinoma". Applied Immunohistochemistry & Molecular Morphology. doi:10.1097/PAI.0000000000000397. ISSN 1533-4058. PMID 27258565.
  8. Nowinska, Katarzyna; Chmielewska, Magdalena; Piotrowska, Aleksandra; Pula, Bartosz; Pastuszewski, Wojciech; Krecicki, Tomasz; Podhorska-Okołow, Marzena; Zabel, Maciej; Dziegiel, Piotr (2016-02-01). "Correlation between levels of expression of minichromosome maintenance proteins, Ki-67 proliferation antigen and metallothionein I/II in laryngeal squamous cell cancer". International Journal of Oncology. 48 (2): 635–645. doi:10.3892/ijo.2015.3273. ISSN 1791-2423. PMID 26648405.


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