Cell junction

Cell junction
Details
Identifiers
Latin junctiones cellulares
TH H1.00.01.0.00012
FMA 67394

Anatomical terminology

A cell junction (or intercellular bridge[1]) is a type of structure that exists within the tissue of some multicellular organisms, such as animals. Cell junctions consist of multiprotein complexes that provide contact between neighboring cells or between a cell and the extracellular matrix. They also build up the paracellular barrier of epithelia and control the paracellular transport. Cell junctions are especially abundant in epithelial tissues.

Cell junctions are especially important in enabling communication between neighboring cells via specialized proteins called communicating junctions. Cell junctions are also important in reducing stress placed upon cells.

Types

Some examples of cell junctions
Some examples of cell junctions

In vertebrates, there are three major types of cell junction:

Invertebrates have several other types of specific junctions, for example septate junctions or the C. elegans apical junction.

In multicellular plants, the structural functions of cell junctions are instead provided for by cell walls. The analogues of communicative cell junctions in plants are called plasmodesmata.

Anchoring junctions

Cells within tissues and organs must be anchored to one another and attached to components of the extracellular matrix. Cells have developed several types of junctional complexes to serve these functions, and in each case, anchoring proteins extend through the plasma membrane to link cytoskeletal proteins in one cell to cytoskeletal proteins in neighboring cells as well as to proteins in the extracellular matrix .[3]

Three types of anchoring junctions are observed, and differ from one another in the cytoskeletal protein anchor as well as the transmembrane linker protein that extends through the membrane:

Junction Cytoskeletal anchor Transmembrane linker Ties cell to:
Desmosomes Intermediate filaments Cadherin Other cells
Hemidesmosomes Intermediate filaments Integrins EC matrix
Adherens junctions Actin filaments Cadherin / Integrins Other cells / EC matrix

Anchoring-type junctions not only hold cells together but provide tissues with structural cohesion. These junctions are most abundant in tissues that are subject to constant mechanical stress such as skin and heart.[3]

Desmosomes

This image shows a desmosome junction between cells of the epidermal layer of the skin.

Desmosomes, also termed as maculae adherentes, can be visualized as rivets through the plasma membrane of adjacent cells. Intermediate filaments composed of keratin or desmin are attached to membrane-associated attachment proteins that form a dense plaque on the cytoplasmic face of the membrane. Cadherin molecules form the actual anchor by attaching to the cytoplasmic plaque, extending through the membrane and binding strongly to cadherins coming through the membrane of the adjacent cell.[4]

Hemidesmosomes

Hemidesmosomes form rivet-like links between cytoskeleton and extracellular matrix components such as the basal laminae that underlie epithelia. Like desmosomes, they tie to intermediate filaments in the cytoplasm, but in contrast to desmosomes, their transmembrane anchors are integrins rather than cadherins.[5]

Adherens Junctions

Adherens junctions share the characteristic of anchoring cells through their cytoplasmic actin filaments. Similarly to desmosomes and hemidesmosomes, their transmembrane anchors are composed of cadherins in those that anchor to other cells and integrins in those that anchor to extracellular matrix. There is considerable morphologic diversity among adherens junctions. Those that tie cells to one another are seen as isolated streaks or spots, or as bands that completely encircle the cell. The band-type of adherens junctions is associated with bundles of actin filaments that also encircle the cell just below the plasma membrane. Spot-like adherens junctions help cells adhere to extracellular matrix both in vivo and in vitro where they are called focal adhesions. The cytoskeletal actin filaments that tie into adherens junctions are contractile proteins and in addition to providing an anchoring function, adherens junctions are thought to participate in folding and bending of epithelial cell sheets. Thinking of the bands of actin filaments as being similar to 'drawstrings' allows one to envision how contraction of the bands within a group of cells would distort the sheet into interesting patterns[3]

Communicating (GAP) junctions

Communicating junctions, or gap junctions allow for direct chemical communication between adjacent cellular cytoplasm through diffusion without contact with the extracellular fluid.[6] This is possible due to six connexin proteins interacting to form a cylinder with a pore in the centre called a connexon.[7] The connexon complexes stretches across the cell membrane and when two adjacent cell connexons interact, they form a complete gap junction channel.[6][7] Connexon pores vary in size, polarity and therefore can be specific depending on the connexin proteins that constitute each individual connexon.[6][7] Whilst variation in gap junction channels do occur, their structure remains relatively standard, and this interaction ensures efficient communication without the escape of molecules or ions to the extracellular fluid.[7]

Gap junctions play vital roles in the human body,[8] including their role in the uniform contractile of the heart muscle.[8] They are also relevant in signal transfers in the brain, and their absence shows a decreased cell density in the brain.[9] Retinal and skin cells are also dependent on gap junctions in cell differentiation and proliferation.[8][9]

Tight junctions

Found in vertebrate epithelia, tight junctions act as barriers that regulate the movement of water and solutes between epithelial layers. Tight junctions are classified as a paracellular barrier which is defined as not having directional discrimination; however, movement of the solute is largely dependent upon size and charge. There is evidence to suggest that the structures in which solutes pass through are somewhat like pores.

Physiological pH plays a part in the selectivity of solutes passing through tight junctions with most tight junctions being slightly selective for cations. Tight junctions present in different types of epithelia are selective for solutes of differing size, charge, and polarity.

Proteins

There have been approximately 40 proteins identified to be involved in tight junctions. These proteins can be classified into four major categories; scaffolding proteins, signalling proteins, regulation proteins, and transmembrane proteins.

Roles of Tight Junction Proteins

Scaffolding Proteins — organise the transmembrane proteins, couple transmembrane proteins to other cytoplasmic proteins as well as to actin filaments.

Signaling Proteins — involved in junctions assembly, barrier regulation, and gene transcription.

Regulation Proteins — regulate membrane vesicle targeting.

Transmembrane Proteins — including junctional adhesion molecule (JAM), occludin, and claudin. It is believed that claudin is the protein molecule responsible for the selective permeability between epithelial layers.

A three-dimensional image is still yet to be achieved and as such specific information about the function of tight junctions is yet to be determined.

Tricellular junctions (TCJ)

Tricellular junctions (TCJ) seal epithelia at the corners of three cells. Due to the geometry of three-cell vertices, the sealing of the cells at these sites requires a specific junctional organization, different from bicellular junctions. In vertebrates, TCJs components are Tricellulin and lipolysis-stimulated lipoprotein receptors. In invertebrates TCJs components are Gliotactin and Anaconda.[10]

The cartoon of epithelium cells connected by tri-cellular-junctions (TCJ) at the regions where three cells meet.

Additionally to the barrier functions, TCJ are also implicated in the regulation of cytoskeletal organization and cell divisions. In particular they ensure that cells divide according to the Hertwig rule. In Drosophila epithelium during cell divisions TCJs establish physical contact with spindle apparatus through astral microtubules. TJCs excert pulling force on the spindle apparatus and serve as a geomentrical clues to determine orientation of cell divisions.[11]

Cell junction molecules

The molecules responsible for creating cell junctions include various cell adhesion molecules. There are four main types: selectins, cadherins, integrins, and the immunoglobulin superfamily.[12]

Selectins are cell adhesion molecules that play an important role in the initiation of inflammatory processes.[13] The functional capacity of selectin is limited to leukocyte collaborations with vascular endothelium. There are three types of selectins found in humans; L-selectin, P-selectin and E-selectin. L-selectin deals with lymphocytes, monocytes and neutrophils, P-selectin deals with platelets and endothelium and E-selectin deals only with endothelium. They have extracellular regions made up of an amino-terminal lectin domain, attached to a carbohydrate ligand, growth factor-like domain (EGF) and short repeat units (numbered circles) that match the complimentary binding protein domains.[14]

Cadherins are calcium-dependent adhesion molecules. Cadherins are extremely important in the process of morphogenesis – fetal development. Together with an alpha-beta catenin complex, the cadherin can bind to the microfilaments of the cytoskeleton of the cell. This allows for homophilic cell–cell adhesion.[15] The β-catenin–α-catenin linked complex at the adherens junctions allows for the formation of a dynamic link to the actin cytoskeleton.[16]

Integrins act as adhesion receptors, transporting signals across the plasma membrane in multiple directions. These molecules are an invaluable part of cellular communication, as a single ligand can be used for many integrins. Unfortunately these molecules still have a long way to go in the ways of research.[17]

Immunoglobulin superfamily are a group of calcium independent proteins capable of homophilic and heterophilic adhesion. Homophilic adhesion involves the immunoglobulin-like domains on the cell surface binding to the immunoglobulin-like domains on an opposing cell’s surface while heterophilic adhesion refers to the binding of the immunoglobulin-like domains to integrins and carbohydrates instead.[18]

Cell adhesion is a vital component of the body. Loss of this adhesion effects cell structure, cellular functioning and communication with other cells and the extracellular matrix and can lead to severe health issues and diseases.

References

  1. ↑ Mitchell, Richard Sheppard; Kumar, Vinay; Abbas, Abul K.; Fausto, Nelson. "Ch. 13: Box on morphology of squamous cell carcinoma". Robbins Basic Pathology (8th ed.). Philadelphia: Saunders. ISBN 1-4160-2973-7.
  2. ↑ Andrew L Harris; Darren Locke (2009). Connexins, A Guide. New York: Springer. p. 574. ISBN 978-1-934115-46-6.
  3. 1 2 3 Yan HH, Mruk DD, Lee WM, Cheng CY (2008). "Cross-talk between tight and anchoring junctions-lesson from the testis". Adv. Exp. Med. Biol. 636: 234–54. doi:10.1007/978-0-387-09597-4_13. PMID 19856171.
  4. ↑ Lie PP, Cheng CY, Mruk DD (2011). "The biology of the desmosome-like junction a versatile anchoring junction and signal transducer in the seminiferous epithelium". Int Rev Cell Mol Biol. 286: 223–69. doi:10.1016/B978-0-12-385859-7.00005-7. PMID 21199783.
  5. ↑ Gipson IK, Spurr-Michaud SJ, Tisdale AS (April 1988). "Hemidesmosomes and anchoring fibril collagen appear synchronously during development and wound healing". Dev. Biol. 126 (2): 253–62. doi:10.1016/0012-1606(88)90136-4. PMID 3350210.
  6. 1 2 3 Evans WH, Martin PE (2002). "Gap junctions: structure and function (Review)". Mol. Membr. Biol. 19 (2): 121–36. doi:10.1080/09687680210139839. PMID 12126230.
  7. 1 2 3 4 Lampe PD, Lau AF (July 2004). "The effects of connexin phosphorylation on gap junctional communication". Int. J. Biochem. Cell Biol. 36 (7): 1171–86. doi:10.1016/S1357-2725(03)00264-4. PMC 2878204Freely accessible. PMID 15109565.
  8. 1 2 3 "Abstracts: Proceedings of the International Gap Junction Conference. August 5–9, 2007. Elsinore, Denmark". Cell Commun. Adhes. 14 (6): 275–346. 2007. doi:10.1080/15419060801891042. PMID 18392995.
  9. 1 2 Wei CJ, Xu X, Lo CW (2004). "Connexins and cell signaling in development and disease". Annu. Rev. Cell Dev. Biol. 20: 811–38. doi:10.1146/annurev.cellbio.19.111301.144309. PMID 15473861.
  10. ↑ Byri S, Misra T, Syed ZA, Batz T, Shah J, Boril L, Glashauser J, Aegerter-Wilmsen T, Matzat T, Moussian B, Uv A, Luschnig S (2015). "The triple-repeat protein Anakonda controls epithelial tricellular junction formation in Drosophila.". Developmental Cell. 33 (5): 535–48.
  11. ↑ Bosveld F, Markova O, Guirao B, Martin C, Wang Z, Pierre A, Balakireva M, Gaugue I, Ainslie A, Christophorou N, Lubensky DK, Minc N, Bellaïche Y (2016). "Epithelial tricellular junctions act as interphase cell shape sensors to orient mitosis.". Nature. 25 (530): 496–8. PMID 26886796.
  12. ↑ Lodish; et al. (2007). Molecular Cell Biology (6th ed.). W. H. Freeman and Company. p. 803. ISBN 1429203145.
  13. ↑ Tedder TF, Steeber DA, Chen A, Engel P (July 1995). "The selectins: vascular adhesion molecules". FASEB J. 9 (10): 866–73. PMID 7542213.
  14. ↑ Bevilacqua MP, Nelson RM (February 1993). "Selectins". J. Clin. Invest. 91 (2): 379–87. doi:10.1172/JCI116210. PMC 287934Freely accessible. PMID 7679406.
  15. ↑ Rowlands TM, Symonds JM, Farookhi R, Blaschuk OW (January 2000). "Cadherins: crucial regulators of structure and function in reproductive tissues". Rev. Reprod. 5 (1): 53–61. doi:10.1530/revreprod/5.1.53. PMID 10711736.
  16. ↑ Brembeck FH, Rosário M, Birchmeier W (February 2006). "Balancing cell adhesion and Wnt signaling, the key role of β-catenin". Curr. Opin. Genet. Dev. 16 (1): 51–9. doi:10.1016/j.gde.2005.12.007. PMID 16377174.
  17. ↑ Hynes RO (September 2002). "Integrins: bidirectional, allosteric signaling machines". Cell. 110 (6): 673–87. doi:10.1016/S0092-8674(02)00971-6. PMID 12297042.
  18. ↑ Wai Wong C, Dye DE, Coombe DR (2012). "The role of immunoglobulin superfamily cell adhesion molecules in cancer metastasis". Int J Cell Biol. 2012: 340296. doi:10.1155/2012/340296. PMC 3261479Freely accessible. PMID 22272201.

3. From CCH(2010)

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