UvrABC endonuclease

UvrABC endonuclease is a multienzyme complex in Escherichia coli involved in DNA repair by nucleotide excision repair, and it is, therefore, sometimes called an excinuclease. This UvrABC repair process, sometimes called the short-patch process, involves the removal of twelve nucleotides where a genetic mutation has occurred followed by a DNA polymerase, replacing these aberrant nucleotides with the correct nucleotides and completing the DNA repair. The subunits for this enzyme are encoded in the uvrA, uvrB, and uvrC genes. This enzyme complex is able to repair many different types of damage, including cyclobutyl dimer formation.[1]

Mechanism

  1. Two UvrA proteins form a dimer and they both have ATPase/GTPase activity.
  2. The UvrA dimer binds with a UvrB dimer[2] and forms a complex that is able to detect DNA damage.The UvrA dimer functions as the unit responsible for the detection of DNA damage, probably through a mechanism of detecting distortions in the DNA double helix.
  3. Upon binding of the UvrA2B2 complex to a putative damaged site, the DNA wraps around UvrB[3]
  4. The UvrA dimer leaves and a UvrC protein comes in and binds to the UvrB and, hence, forms a new UvrBC complex.
  5. UvrC is responsible for cleaving the nucleotides either side of the DNA damage.[4] It cleaves a phosphodiester bond four nucleotides downstream of the DNA damage, and cleaves a phosphodiester bond eight nucleotides upstream of the DNA damage and creates a twelve nucleotide excised segment.
  6. DNA helicase II (sometimes called UvrD) then comes in and removes the excised segment by removing the base pairing. The UvrB still remains in place even though UvrC has disassociated at this stage, as UvrB may be involved to prevent the reannealing of the excised DNA.
  7. DNA polymerase I comes in and fills in the correct nucleotides sequence, kicking off UvrB in the process, and the last phosphodiester bond is completed by DNA ligase.

See also

References

  1. ↑ Grossman L, Yeung AT (1990). "The UvrABC endonuclease system of Escherichia coli--a view from Baltimore". Mutat Res. 236 (2-3): 213–21. doi:10.1016/0921-8777(90)90006-q. PMID 2144612.
  2. ↑ Verhoeven, Esther E. A.; Wyman, Claire; Moolenaar, Geri F.; Goosen, Nora (2002-08-01). "The presence of two UvrB subunits in the UvrAB complex ensures damage detection in both DNA strands". The EMBO journal. 21 (15): 4196–4205. doi:10.1093/emboj/cdf396. ISSN 0261-4189. PMC 126143Freely accessible. PMID 12145219.
  3. ↑ "uvrB - UvrABC system protein B - Escherichia coli (strain K12) - uvrB gene & protein". www.uniprot.org. Retrieved 2016-02-28.
  4. ↑ Verhoeven, E. E.; van Kesteren, M.; Moolenaar, G. F.; Visse, R.; Goosen, N. (2000-02-18). "Catalytic sites for 3' and 5' incision of Escherichia coli nucleotide excision repair are both located in UvrC". The Journal of Biological Chemistry. 275 (7): 5120–5123. doi:10.1074/jbc.275.7.5120. ISSN 0021-9258. PMID 10671556.


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