Roswell Park Memorial Institute medium
Roswell Park Memorial Institute medium, commonly referred to as RPMI medium, is a form of medium used in cell culture and tissue culture. It has traditionally been used for growth of human lymphoid cells. This medium contains a great deal of phosphate and is formulated for use in a 5% carbon dioxide atmosphere. RPMI 1640 has traditionally been used for the serum-free expansion of human lymphoid cells.
RPMI 1640 uses a bicarbonate buffering system and differs from most mammalian cell culture media in its typical pH 8 formulation.
Properly supplemented with serum or an adequate serum replacement, RPMI 1640 allows the cultivation of many cell types, especially human T/B-lymphocytes, bone marrow cells and hybridoma cells.
There are a variety of similar media in the RPMI series, such as RPMI 1640.
Roswell Park Surgeon Dr. Roswell Park is best known for starting Gratwick Research Laboratory in 1898, which is now known as Roswell Park Cancer Institute. Wikipedia Born: May 4, 1852, Pomfret, CT Died: February 15, 1914, Buffalo, NY Organization founded: Roswell Park Cancer Institute
The original RPMI-1640 formulation published by [1]
list of ingredients in 1640 :[2]
References
- ↑ Moore G.E. et al. (1967) J.A.M.A. 199,519 PMID 4960081
- ↑ https://www.applichem.com/fileadmin/produktinfo/a2040_en.pdf
External links
- http://www.bioind.com/RPMI1640
- http://www.sigmaaldrich.com/Area_of_Interest/Life_Science/Cell_Culture/Product_Lines/Classic_Media___Salts/RPMI_Media.html
- http://www.invitrogen.com/site/us/en/home/Products-and-Services/Applications/Cell-Culture/Mammalian-Cell-Culture/Classical_Media/RPMI.html