para-Nitrophenylphosphate
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Names | |||
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IUPAC name
(4-nitrophenyl) dihydrogen phosphate | |||
Other names
pNPP | |||
Identifiers | |||
330-13-2 | |||
3D model (Jmol) | Interactive image | ||
ChEBI | CHEBI:17440 | ||
ChEMBL | ChEMBL24231 | ||
ChemSpider | 369 | ||
ECHA InfoCard | 100.005.777 | ||
PubChem | 378 | ||
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Properties | |||
C6H6NO6P | |||
Molar mass | 219.088701 | ||
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa). | |||
verify (what is ?) | |||
Infobox references | |||
para-Nitrophenylphosphate (pNPP) is a chromogenic substrate for acid and alkaline phosphatase in ELISA and conventional spectrophotometric assays. These enzymes catalyse the hydrolysis of pNPP to yellow para-nitrophenol. This product absorbs strongly at 405 nm and can be measured with a visible light spectrophotometer.
External links
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