Nitroprusside reaction

The nitroprusside reaction is a chemical test used for detecting the presence of free thiol groups of cysteine in proteins. Proteins with the free thiol group give a red colour when added to sodium nitroprusside with ammonium hydroxide. Some proteins test positive when denatured, indicating that thiol groups are liberated.[1][2][3] The nitroprusside reaction is usually used for the identification of ketones in urine testing.[4]

References

  1. ↑ Chatterjea (1 January 2004). Textbook of Biochemistry for Dental/Nursing/Pharmacy Students. Jaypee Brothers Publishers. p. 51. ISBN 978-81-8061-204-6.
  2. ↑ Debajyoti Das (1980). Biochemistry. Academic Publishers. p. 56. ISBN 978-93-80599-17-5.
  3. ↑ R.A. Joshi (2006). Question Bank of Biochemistry. New Age International. p. 64. ISBN 978-81-224-1736-4.
  4. ↑ Jerry Kaneko, Jiro; Harvey, John; Bruss, Michael (2008). Clinical Biochemistry of Domestic Animals. Academic Press. p. 98. ISBN 9780080568829.


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