DNA2.0
 | |
| Private | |
| Industry | Biotechnology |
| Founded | 2003 |
| Founders | Sridhar Govindarajan, Claes Gustafsson, Jeremy Minshull, Jon Ness |
| Headquarters | Newark, California, U.S. |
| Products | Gene Synthesis, Protein Engineering, Protein Production, Bioinformatics |
| Website | www.DNA20.com |
DNA2.0 provides products and services for life science and Synthetic biology research. DNA2.0 also provides free access to research tools such as Gene Designer, DNA Atlas and a gRNA designer.
The company
DNA2.0 was founded in 2003, in Menlo Park, California. The company is privately held[1] and continues to have all research, development and production in California, currently in their 50,000 sq ft Newark facility.[2] It began and continues as a gene synthesis and protein engineering provider to academia, government and the pharmaceutical, chemical, agricultural and biotechnology industries. Gene Synthesis rapidly replaced molecular cloning for many academic and corporate labs, as "foundries for the biotechnology age" allowing made-to-order genes for biological research.[3] DNA2.0 was featured on the PBS show Nova ScienceNow[4] to show how genes are created synthetically in a lab. In 2008, the company supplied some of the DNA stretches used to create a synthetic bacterial genome.[5] Dan Rather Reports included DNA2.0 in their episode on Synthetic Biology[6] and how it is solving "some of the most important problems facing the world."[6] In 2009, The Scientist named the codon design algorithms[7] (now trademarked as GeneGPS) developed by DNA2.0 as one of the Top 10 Innovations of the year for Life Sciences.[8] DNA2.0 developed the Electra Vector System, a universal cloning system that utilizes the type IIS restriction enzyme SapI and T4 DNA ligase in a single-tube reaction.[9] DNA2.0 has made some molecular components, such as synthetic fluorescent proteins, available in open-access collections of DNA parts (BioBricks Foundation).[10] DNA2.0 is a founding member of the International Gene Synthesis Consortium (IGSC) to promote biosecurity in the gene-synthesis industry.[11][12] There are over 1,200[13] published scientific articles using DNA2.0 products and/or services, of which 44[14] include company employees as an author(s).
Research Tools
- Gene Designer is a free bioinformatics software package.[15][16] It is used by molecular biologists to design,[17] clone and validate genetic sequences.[18]
- A free gRNA design tool with scoring algorithms for CRISPR.
- DNA ATLAS is a free plasmid mapping tool to show features such as promoters, markers, restriction sites and open reading frames in any DNA vector sequence.
- The online Bioinformatics toolbox was selected as a Best of the Web by Genetic Engineering News.[19]
Partnerships
- The Perelman School of Medicine at the University of Pennsylvania will use the GeneGPS technology developed by DNA2.0 primarily to support the Gene Therapy Program’s work on HIV-1 vaccine development by optimizing in vivo protein expression.[20][21]
- The Infection and Immunity Research Centre at St. George’s, University of London and DNA2.0 partnered to develop plant-based pharmaceuticals.[22]
- Adimab LLC. and DNA2.0 employ an alliance in the area of antibody discovery and biomanufacturing via the design and construction of antibody libraries.
- Cytovance Biologics utilizes GeneGPS from DNA2.0 as part of their Keystone Expression System for microbial strain development for successful biomanufacturing.
- Archer Daniels Midland Company applies DNA2.0's proprietary protein engineering technology, ProteinGPS, to ADM’s industrial enzyme engineering processes.[23]
- DNA2.0 is a sponsor of BioBuilder, a resource for hands-on activities and informative animations on synthetic biology for middle school and high school students and teachers; founded by Dr. Natalie Kuldell at MIT.
Patents
US 9290552, Minshull & Theodorou, "Fluorescent and colored proteins and methods for using them", issued March 22, 2016
US 9206433, Minshull; Ness & Theodorou, "Methods, compositions and kits for a one-step DNA cloning system", issued December 8, 2015
US 9102944, Ness & Minshull, "Methods, compositions and kits for one-step DNA cloning using DNA topoisomerase", issued August 11, 2015
US 8975042, Minshull & Theodorou, "Fluorescent and colored proteins and methods for using them", issued March 10, 2015
US 8825411, Govindarajan; Minshull & Ness, "Design, synthesis and assembly of synthetic nucleic acids", issued September 2, 2014
US 8635029, Gustafsson; Govindarajan & Minshull, "Systems and methods for biopolymer engineering", issued January 21, 2014
US 8412461, Gustafsson; Govindarajan & Minshull, "Systems and methods for antibody engineering", issued April 2, 2013
US 8401798, Welch & Gustafsson, "Systems and methods for constructing frequency lookup tables for expression systems", issued March 19, 2013
US 8323930, Ness & Minshull, "Methods, compositions and kits for one-step DNA cloning using DNA topoisomerase", issued December 4, 2012
US 8158391, Gross; Lu & Ness et al., "Production of an .alpha.-carboxyl-.omega.-hydroxy fatty acid using a genetically modified Candida strain", issued September 28, 2010
US 8126653, Welch & Gustafsson, "Synthetic nucleic acids for expression of encoded proteins", issued February 28, 2012
US 8005620, Gustafsson; Govindarajan & Minshull, "Systems and methods for biopolymer engineering", issued August 23, 2011
US 7805252, Gustafsson; Govindarajan & Ness et al., "Systems and methods for designing and ordering polynucleotides", issued September 28, 2010
US 7561973, Welch & Gustafsson, "Methods for determining properties that affect an expression property value of polynucleotides in an expression system", issued July 14, 2009
US 7561972, Welch & Gustafsson, "Synthetic nucleic acids for expression of encoded proteins", issued July 14, 2009
References
- ↑ Duan, Mary (October 18, 2009). "Gene synthesis: It's no strain for DNA 2.0". Silicon Valley Business Journal. San Jose, CA.
- ↑ "DNA2.0 Builds State of the Art Bioproduction Facility in Newark, California". MercuryNews.com. The San Jose Mercury News. Retrieved 22 March 2016.
- ↑ Pollack (September 12, 2007). "How Do You Like Your Genes? Biofabs Take Orders". The New York Times. New York.
- ↑ "Artificial Life". Nova ScienceNow. October 18, 2005. PBS.
- ↑ Pollack (January 24, 2008). "Scientists Take New Step Toward Man-Made Life". The New York Times. New York.
- 1 2 "Cutting Edge". Dan Rather Reports. April 30, 2013. AXS TV.
- ↑ Welch; Govindarajan; Ness; Villalobos; Gurney; Minshull; Gustafsson (September 14, 2009). "Design parameters to control synthetic gene expression in Escherichia coli.". PLOS ONE. 4 (9): e7002. doi:10.1371/journal.pone.0007002. PMC 2736378
. PMID 19759823. - ↑ "2009 Top 10 Innovations". The Scientist. 23 (12): 41–52. December 1, 2009.
- ↑ Whitman; Gore; Ness; Theodorou; Minshull (June 2013), "Rapid, Scarless Cloning of Gene Fragments Using SapI, T4 DNA Ligase and the DNA2.0 Electra Vector System" (PDF), NEB Expressions, II: 8
- ↑ Ledford, Heidi (July 4, 2013). "Bioengineers Look Beyond Patents". Nature. Macmillan Publishers Limited. 499: 16–17. doi:10.1038/499016a.
- ↑ Hayden, Erika Check (November 18, 2009). "Gene-makers form security coalition". Nature. doi:10.1038/news.2009.1095.
- ↑ "Gene-Synthesis Firms Set Up Biosecurity Protocol", Genetic Engineering & Biotechnology News, November 18, 2009
- ↑ "Literature Database Search All". April 29, 2016.
- ↑ "Literature Database Search Author Affiliation:DNA2.0". April 29, 2016.
- ↑ Villalobos, Alan; Ness, Jon E; Gustafsson, Claes; Minshull, Jeremy; Govindarajan, Sridhar (2006). "Gene Designer: A synthetic biology tool for constructing artificial DNA segments". BMC Bioinformatics. 7: 285. doi:10.1186/1471-2105-7-285. PMC 1523223. PMID 16756672.
- ↑ Villalobos, Alan; Welch, Mark; Minshull, Jeremy (2012). "In Silico Design of Functional DNA Constructs". Gene Synthesis. Methods in Molecular Biology. 852: 197–213. doi:10.1007/978-1-61779-564-0_15. ISBN 978-1-61779-563-3. PMID 22328435.
- ↑ Welch, M; Villalobos, A; Gustafsson, C; Minshull, J (2011). "Designing genes for successful protein expression". Methods in enzymology. 498: 43–66. doi:10.1016/B978-0-12-385120-8.00003-6. PMID 21601673.
- ↑ Dance, Amber (July 1, 2012). "Mover Over, Mother Nature.". The Scientist.
- ↑ "Best of the Web". Genetic Engineering News. April 15, 2007.
- ↑ "Optimizing Gene Expression with DNA2.0 Technology.". GEN. November 14, 2012.
- ↑ McEnery, Regina (February 4, 2013), "Delivering the DNA", VAX The Bulletin on AIDS Vaccine Research, 11 (1)
- ↑ Cohen, Bryan (December 24, 2013). "DNA2.0 Announces Biopharmaceutical Partnership with St. George's". Vaccine News.
- ↑ "ADM and DNA2.0 Enter Into Protein Engineering Technology Access and Service Agreement.". San Jose Mercury News. San Jose, CA. November 17, 2014.