Colicin

Colicin

structure of tolb in complex with a peptide of the colicin e9 t-domain
Identifiers
Symbol Colicin
Pfam PF03515
Pfam clan CL0446
InterPro IPR003058
SCOP 1jch
SUPERFAMILY 1jch

A colicin is a type of bacteriocin produced by and toxic to some strains of Escherichia coli.[1] Colicins are released into the environment to reduce competition from other bacterial strains. Colicins bind to outer membrane receptors, using them to translocate to the cytoplasm or cytoplasmic membrane, where they exert their cytotoxic effect, including depolarisation of the cytoplasmic membrane, DNase activity, RNase activity, or inhibition of murein synthesis.

Structure

Channel-forming colicins (colicins A, B, E1, Ia, Ib, and N) are transmembrane proteins that depolarize the cytoplasmic membrane, leading to dissipation of cellular energy.[2] These colicins contain at least three domains: an N-terminal translocation domain responsible for movement across the outer membrane and periplasmic space; a central domain responsible for receptor recognition; and a C-terminal cytotoxic domain responsible for channel formation in the cytoplasmic membrane.[3][4] One domain regulates the target and binds to the receptor on the sensitive cell. The second is involved with translocation, co-opting the machinery of the target cell. The third is the 'killing' domain and may produce a pore in the target cell membrane, or act as a nuclease to chop up the DNA or RNA of the target cell.

Translocation

Most colicins are able to translocate the outer membrane by a two-receptor system, where one receptor is used for the initial binding and the second for translocation. The initial binding is to cell surface receptors such as the outer membrane proteins OmpF, FepA, BtuB, Cir and FhuA; colicins have been classified according which receptors they bind to. The presence of specific periplasmic proteins, such as TolA, TolB, TolC, or TonB, are required for translocation across the membrane.[5] Cloacin DF13 is a bacteriocin that inactivates ribosomes by hydrolysing 16S RNA in 30S ribosomes at a specific site.[6]

Resistance

Because they target specific receptors and use specific translocation machinery, cells can make themselves resistant to the colicin by repressing or deleting the genes for these proteins. Such resistant cells may suffer the lack of a key nutrient (such as iron or a B vitamin), but benefit by not being killed. Colicins exhibit a '1-hit killing kinetic' which does not necessarily mean a single molecule is sufficient to kill, but certainly that it only takes a small number. In his 1969 Nobel Laureate speech, Salvador E. Luria speculated that colicins could only be this toxic by causing a domino effect that destabilized the cell membrane.[7] He was not entirely correct, but pore-forming colicins do depolarize the membrane and thus eliminate the energy source for the cell. The colicins are highly effective toxins.

Genetic organisation

Virtually all colicins are carried on plasmids. The two general classes of colicinogenic plasmids are large, low-copy-number plasmids, and small, high-copy-number plasmids. The larger plasmids carry other genes, as well as the colicin operon. The colicin operons are generally organized with several major genes. These include an immunity gene, a colicin structural gene, and a bacteriocin release protein (BRP), or lysis, gene. The immunity gene is often produced constitutively, while the BRP is generally produced only as a read-through of the stop codon on the colicin structural gene. The colicin itself is repressed by the SOS response and may be regulated in other ways, as well.

Retaining the colicin plasmid is very important for cells that live with their relatives, because if a cell loses the immunity gene, it quickly becomes subject to destruction by circulating colicin. At the same time, colicin is only released from a producing cell by the use of the lysis protein, which results in that cell's death. This suicidal production mechanism would appear to be very costly, except for the fact that it is regulated by the SOS response, which responds to significant DNA damage. In short, colicin production may only occur in terminally ill cells. The Professor Kleanthous Research Group at the University of York study colicins extensively as a model system for characterising and investigating protein-protein interactions and recognition.[8]

BACTIBASE[9][10] database is an open-access database for bacteriocins including colicins (view complete list).

References

  1. ↑
    • Feldgarden, M., & Riley, M. A. (August 1999). The phenotypic and fitness effects of colicin resistance in Escherichia coli K-12" Evolution 53(4), 1019-27.
  2. ↑ Kang C, Postle K, Chen G, Park H, Youn B, Hilsenbeck JL (2004). "Crystal structure of the cytotoxic bacterial protein colicin B at 2.5 A resolution". Mol. Microbiol. 51 (3): –. doi:10.1111/j.1365-2958.2003.03884.x. PMID 14731273.
  3. ↑ Cramer WA, Zakharov SD, Antonenko YN, Kotova EA (2004). "On the role of lipid in colicin pore formation". Biochim. Biophys. Acta. 1666 (1): –. doi:10.1016/j.bbamem.2004.07.001. PMID 15519318.
  4. ↑ Cascales et al. (2007). Colicin Biology. Microbio. and Mol. Bio. Rev. 71(1), 158-229. Abstractpdf
  5. ↑ Cao Z, Klebba PE (2002). "Mechanisms of colicin binding and transport through outer membrane porins". Biochimie. 84 (5-6): 399–412. doi:10.1016/S0300-9084(02)01455-4. PMID 12423783.
  6. ↑ van den Elzen PJ, Veltkamp E, Nijkamp HJ, Walters HH (1983). "Molecular structure and function of the bacteriocin gene and bacteriocin protein of plasmid Clo DF13". Nucleic Acids Res. 11 (8): 2465–2477. doi:10.1093/nar/11.8.2465. PMC 325896Freely accessible. PMID 6344017.
  7. ↑ Luria, S. E. (1969) Nobel Lecture
  8. ↑ "Kleanthous Research Group". University of York. Retrieved 11 April 2011.
  9. ↑ Hammami R, Zouhir A, Ben Hamida J, Fliss I (2007). "BACTIBASE: a new web-accessible database for bacteriocin characterization". BMC Microbiology. 7: 89. doi:10.1186/1471-2180-7-89. PMC 2211298Freely accessible. PMID 17941971.
  10. ↑ Hammami R, Zouhir A, Le Lay C, Ben Hamida J, Fliss I (2010). "BACTIBASE second release: a database and tool platform for bacteriocin characterization.". BMC Microbiology. 10: 22. doi:10.1186/1471-2180-10-22. PMC 2824694Freely accessible. PMID 20105292.

External links

This article incorporates text from the public domain Pfam and InterPro IPR000290

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