Cocaine esterase

Cocaine esterase (EC 3.1.1.84, CocE, hCE2, hCE-2, human carboxylesterase 2) is an enzyme with systematic name cocaine benzoylhydrolase.^[1]^[2]^[3]^[4]^[5] This enzyme catalyses the following chemical reaction

cocaine + H₂O

\rightleftharpoons

ecgonine methyl ester + benzoate

Rhodococcus sp. strain MB1 and Pseudomonas maltophilia strain MB11L can utilize cocaine as sole source of carbon and energy.

References

↑ Gao, D.; Narasimhan, D.L.; Macdonald, J.; Brim, R.; Ko, M.C.; Landry, D.W.; Woods, J.H.; Sunahara, R.K.; Zhan, C.G. (2009). "Thermostable variants of cocaine esterase for long-time protection against cocaine toxicity". Mol. Pharmacol. 75 (2): 318–323. doi:10.1124/mol.108.049486. PMID 18987161.
↑ Bresler, M.M.; Rosser, S.J.; Basran, A.; Bruce, N.C. (2000). "Gene cloning and nucleotide sequencing and properties of a cocaine esterase from Rhodococcus sp. strain MB1". Appl. Environ. Microbiol. 66 (3): 904–908. doi:10.1128/aem.66.3.904-908.2000. PMID 10698749.
↑ Britt, A.J.; Bruce, N.C.; Lowe, C.R. (1992). "Identification of a cocaine esterase in a strain of Pseudomonas maltophilia". J. Bacteriol. 174 (7): 2087–2094. PMID 1551831.
↑ Larsen, N.A.; Turner, J.M.; Stevens, J.; Rosser, S.J.; Basran, A.; Lerner, R.A.; Bruce, N.C.; Wilson, I.A. (2002). "Crystal structure of a bacterial cocaine esterase". Nat. Struct. Biol. 9 (1): 17–21. doi:10.1038/nsb742. PMID 11742345.
↑ Pindel, E.V.; Kedishvili, N.Y.; Abraham, T.L.; Brzezinski, M.R.; Zhang, J.; Dean, R.A.; Bosron, W.F. (1997). "Purification and cloning of a broad substrate specificity human liver carboxylesterase that catalyzes the hydrolysis of cocaine and heroin". J. Biol. Chem. 272: 14769–14775. doi:10.1074/jbc.272.23.14769. PMID 9169443.

3.1.1: Carboxylic
ester hydrolases

Endodeoxyribonuclease	Deoxyribonuclease I Deoxyribonuclease II Deoxyribonuclease IV Restriction enzyme UvrABC endonuclease

Endoribonuclease	RNase III RNase H 1 2A 2B 2C RNase P RNase A 1 2 3 4/5 RNase T1 RNA-induced silencing complex

either deoxy- or ribo-	Aspergillus nuclease S1 Micrococcal nuclease

Enzymes

Activity	Active site Binding site Catalytic triad Oxyanion hole Enzyme promiscuity Catalytically perfect enzyme Coenzyme Cofactor Enzyme catalysis Enzyme kinetics Lineweaver–Burk plot Michaelis–Menten kinetics

Regulation	Allosteric regulation Cooperativity Enzyme inhibitor

Classification	EC number Enzyme superfamily Enzyme family List of enzymes

Types	EC1 Oxidoreductases (list) EC2 Transferases (list) EC3 Hydrolases (list) EC4 Lyases (list) EC5 Isomerases (list) EC6 Ligases (list)

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