ATP5E

ATP5E
Identifiers
Aliases	ATP5E, ATPE, MC5DN3, ATP synthase, H+ transporting, mitochondrial F1 complex, epsilon subunit
External IDs	HomoloGene: 128187 GeneCards: ATP5E
Gene ontology Molecular function • hydrolase activity • transmembrane transporter activity • proton-transporting ATP synthase activity, rotational mechanism • proton-transporting ATPase activity, rotational mechanism • ATPase activity Cellular component • membrane • mitochondrial proton-transporting ATP synthase complex, catalytic core F(1) • proton-transporting ATP synthase complex, catalytic core F(1) • mitochondrial matrix • mitochondrion • mitochondrial inner membrane • mitochondrial proton-transporting ATP synthase complex Biological process • transport • proton transport • ion transport • ATP synthesis coupled proton transport • mitochondrial ATP synthesis coupled proton transport • ATP biosynthetic process Sources:Amigo / QuickGO
RNA expression pattern
More reference expression data
Orthologs
Species	Human	Mouse
Entrez	514	n/a
Ensembl	ENSG00000124172	n/a
UniProt	P56381	n/a
RefSeq (mRNA)	NM_006886	n/a
RefSeq (protein)	NP_008817.1	n/a
Location (UCSC)	Chr 20: 59.03 – 59.03 Mb	n/a
PubMed search	[1]	n/a
Wikidata

Mitochondrial ATP synthase epsilon chain
ground state structure of f1-atpase from bovine heart mitochondria (bovine f1-atpase crystallised in the absence of azide)
Identifiers
Symbol	ATP-synt_Eps
Pfam	PF04627
InterPro	IPR006721
SCOP	1e79
SUPERFAMILY	1e79
Available protein structures: Pfam structures PDB RCSB PDB; PDBe; PDBj PDBsum structure summary

ATP synthase subunit epsilon, mitochondrial is an enzyme that in humans is encoded by the ATP5E gene.^[2][3]

This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F₁, and the membrane-spanning component, F_o, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). This gene encodes the epsilon subunit of the catalytic core. Two pseudogenes of this gene are located on chromosomes 4 and 13.^[3]

Structure

The ATP5E gene, located on the q arm of chromosome 20 in position 13.32, is made up of 3 exons and is 3,690 base pairs in length.^[3] The ATP5A1 protein weighs 5.7 kDa and is composed of 51 amino acids.^[4][5] The protein is a subunit of the F₁F_o ATPase, also known as Complex V, which consists of 14 nuclear and 2 mitochondrial -encoded subunits. As an alpha subunit, ATP5A1 is contained within the catalytic F₁ portion of the complex and acts as a regulatory subunit that inhibits the binding of ADP, preventing wasteful ATP production.^[3] The nomenclature of the enzyme has a long history. The F₁ fraction derives its name from the term "Fraction 1" and F_o (written as a subscript letter "o", not "zero") derives its name from being the binding fraction for oligomycin, a type of naturally-derived antibiotic that is able to inhibit the F_o unit of ATP synthase.^[6][7] The F₁ particle is large and can be seen in the transmission electron microscope by negative staining.^[8] These are particles of 9 nm diameter that pepper the inner mitochondrial membrane. They were originally called elementary particles and were thought to contain the entire respiratory apparatus of the mitochondrion, but, through a long series of experiments, Efraim Racker and his colleagues (who first isolated the F₁ particle in 1961) were able to show that this particle is correlated with ATPase activity in uncoupled mitochondria and with the ATPase activity in submitochondrial particles created by exposing mitochondria to ultrasound. This ATPase activity was further associated with the creation of ATP by a long series of experiments in many laboratories.

Function

Mitochondrial membrane ATP synthase (F₁F_o ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F₁ - containing the extramembraneous catalytic core, and F_o - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F₁ is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F₁ domain and of the central stalk which is part of the complex rotary element. Rotation of the central stalk against the surrounding alpha₃beta₃ subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits (By similarity).^[9]

The epsilon subunit is located in the stalk region of the F₁ complex, and acts as an inhibitor of the ATPase catalytic core. The epsilon subunit can assume two conformations, contracted and extended, where the latter inhibits ATP hydrolysis. The conformation of the epsilon subunit is determined by the direction of rotation of the gamma subunit, and possibly by the presence of ADP. The epsilon subunit is thought to become extended in the presence of ADP, thereby acting as a safety lock to prevent wasteful ATP hydrolysis.^[10]

Clinical significance

Mutations in the ATP5E gene cause mitochondrial complex V deficiency, nuclear 3 (MC5DN3), a mitochondrial disorder with heterogeneous clinical manifestations including dysmorphic features, psychomotor retardation, hypotonia, growth retardation, cardiomyopathy, enlarged liver, hypoplastic kidneys and elevated lactate levels in urine, plasma and cerebrospinal fluid.^[11]

Reduced expression of ATP5E is significantly associated with the diagnosis of Papillary Thyroid Cancer and may serve as an early tumor marker of the disease.^[12] Papillary Thyroid Cancer is the most common type of thyroid cancer,^[13] representing 75 percent to 85 percent of all thyroid cancer cases.^[14] It occurs more frequently in women and presents in the 20–55 year age group. It is also the predominant cancer type in children with thyroid cancer, and in patients with thyroid cancer who have had previous radiation to the head and neck.^[15]

References

Further reading

PDB gallery
1e79: BOVINE F1-ATPASE INHIBITED BY DCCD (DICYCLOHEXYLCARBODIIMIDE)  1h8e: (ADP.ALF4)2(ADP.SO4) BOVINE F1-ATPASE (ALL THREE CATALYTIC SITES OCCUPIED)  2ck3: AZIDE INHIBITED BOVINE F1-ATPASE  2jdi: GROUND STATE STRUCTURE OF F1-ATPASE FROM BOVINE HEART MITOCHONDRIA (BOVINE F1-ATPASE CRYSTALLISED IN THE ABSENCE OF AZIDE)

This article incorporates text from the United States National Library of Medicine, which is in the public domain.

This article incorporates text from the public domain Pfam and InterPro IPR006721