1,8-Cineole 2-endo-monooxygenase

1,8-Cineole 2-endo-monooxygenase (EC 1.14.13.156, P450cin, CYP176A, CYP176A1) is an enzyme with systematic name 1,8-cineole,NADPH:oxygen oxidoreductase (2-endo-hydroxylating).^[1]^[2]^[3]^[4] This enzyme catalyses the following chemical reaction

1,8-cineole + NADPH + H⁺ + O₂

\rightleftharpoons

2-endo-hydroxy-1,8-cineole + NADP⁺ + H₂O

1,8-Cineole 2-endo-monooxygenase is a heme-thiolate protein (P-450).

References

↑ Hawkes, D.B.; Adams, G.W.; Burlingame, A.L.; Ortiz de Montellano, P.R.; De Voss, J.J. (2002). "Cytochrome P450_cin (CYP176A), isolation, expression, and characterization". J. Biol. Chem. 277 (31): 27725–27732. doi:10.1074/jbc.M203382200. PMID 12016226.
↑ Meharenna, Y.T.; Li, H.; Hawkes, D.B.; Pearson, A.G.; De Voss, J.; Poulos, T.L. (2004). "Crystal structure of P450_cin in a complex with its substrate, 1,8-cineole, a close structural homologue to D-camphor, the substrate for P450_cam". Biochemistry. 43 (29): 9487–9494. doi:10.1021/bi049293p. PMID 15260491.
↑ Kimmich, N.; Das, A.; Sevrioukova, I.; Meharenna, Y.; Sligar, S.G.; Poulos, T.L. (2007). "Electron transfer between cytochrome P450_cin and its FMN-containing redox partner, cindoxin". J. Biol. Chem. 282 (37): 27006–27011. doi:10.1074/jbc.M703790200. PMID 17606612.
↑ Meharenna, Y.T.; Slessor, K.E.; Cavaignac, S.M.; Poulos, T.L.; De Voss, J.J. (2008). "The critical role of substrate-protein hydrogen bonding in the control of regioselective hydroxylation in p450cin". J. Biol. Chem. 283 (16): 10804–10812. doi:10.1074/jbc.M709722200. PMC 2447660. PMID 18270198.

Oxidoreductases: dioxygenases, including steroid hydroxylases (EC 1.14)

1.14.11: 2-oxoglutarate	Prolyl hydroxylase Lysyl hydroxylase

1.14.13: NADH or NADPH	Flavin-containing monooxygenase FMO1 FMO2 FMO3 FMO4 FMO5 Nitric oxide synthase NOS1 NOS2 NOS3 Cholesterol 7 alpha-hydroxylase Methane monooxygenase 3A4 Lanosterol 14 alpha-demethylase 24-hydroxycholesterol 7α-hydroxylase

1.14.14: reduced flavin or flavoprotein	19A1 2D6 2E1

1.14.15: reduced iron-sulfur protein	11B1 11B2 11A1

1.14.16: reduced pteridine (BH4 dependent)	Phenylalanine hydroxylase Tyrosine hydroxylase Tryptophan hydroxylase

1.14.17: reduced ascorbate	Dopamine beta-hydroxylase

1.14.18-19: other	Tyrosinase Stearoyl-CoA desaturase-1

1.14.99 - miscellaneous	Cyclooxygenase Heme oxygenase (HMOX1) Squalene monooxygenase 17A1 21A2

Enzymes

Activity	Active site Binding site Catalytic triad Oxyanion hole Enzyme promiscuity Catalytically perfect enzyme Coenzyme Cofactor Enzyme catalysis Enzyme kinetics Lineweaver–Burk plot Michaelis–Menten kinetics

Regulation	Allosteric regulation Cooperativity Enzyme inhibitor

Classification	EC number Enzyme superfamily Enzyme family List of enzymes

Types	EC1 Oxidoreductases (list) EC2 Transferases (list) EC3 Hydrolases (list) EC4 Lyases (list) EC5 Isomerases (list) EC6 Ligases (list)

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